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. 2019 Apr 25;9:6402. doi: 10.1038/s41598-019-42848-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MSC and CS/β-GP hydrogel transplantation. Hematoxylin/eosin staining shows the uninjured rostral spinal level and the lesion/graft site (A); in (B) a higher magnification of the injury level. In blue DAPI nuclei (C-G-K), in green EGFP-positive MSCs (D-H-L), in red astrocytes forming the glial scar at the lesion site (E-I-M) and the three previous images overlapped (F-J-N). In (C–F) the lesion area at low magnification shows astrocyte distributed around the glial cyst (dashed line in E). At higher magnification MSCs are visible around (H) and inside the lesion (L). Scale bar: (A) 300 μm, (B) 100 μm, (C–F) 70 um, (G–N) 40 um.