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. 2019 Apr 25;9:6539. doi: 10.1038/s41598-019-42990-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Knockout of DYRK1A promotes radioresistance and cell survival. (A) Quantification of proliferating HeLa cells by cell cycle phase: flow cytometry analysis of propidium iodide staining and BrdU incorporation; 10 µM BrdU was pulsed 60 minutes prior to harvest. n = 3 (student’s t-test). (B) Quantification of HeLa cells by cell cycle phase 18 hours following 4 Gy of IR: flow cytometry analysis was done using propidium iodide staining of either WT or DYRK1A KO HeLa cells. n = 3 (student’s t-test). (C) Results of clonogenic survival assay show increased radioresistance of DYRK1A KO cells above WT survival following 4 Gy of IR. Cell colonies were counted using clono-counter java package 12 days following initial radiation. (D) Kaplan-Meier survival analysis of cervical squamous cell carcinoma tumors generated through KM plotter^74,75. Patients with high DYRK1A expression in their tumors had increased survival probability over those with low DYRK1A expression.