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. 2019 Apr 25;9:6403. doi: 10.1038/s41598-019-42833-3

Figure 1.

Figure 1

Expression levels of key SNARE proteins in hippocampus of wild-type (WT) and SNAP-25b-deficient (MT) mouse brains at 4 weeks of age. (a) There are small amounts of SNAP-25a protein expressed in male hippocampus at 4 weeks of age but the major isoform is SNAP-25b (****p < 0.0001, Student’s t-test, n = 4 in duplicates). (b) Similarly, SNAP-25b is the major isoform in females (****p < 0.0001, Student’s t-test, n = 4 in duplicates). There were no significant differences observed in the expression levels of Syntaxin 1A or SNAP-47 in the hippocampus of either sex at 4 weeks of age. No significant differences were observed in expression levels of VAMP2 between WT and MT males, however, a significantly lower expression of VAMP2 was observed in MT females (*p < 0.05, Student’s t-test, n = 4 in duplicates). (c) The females differed from males in the expression ratios between the ‘a’ and ‘b’ isoforms, having higher levels of the developmentally earlier SNAP-25a isoform remaining at 4 weeks age in hippocampus (***p < 0.01, one-way ANOVA with Bonferroni’s multiple comparison test). (d) No detectable SNAP-25b was expressed in mutant males and females mice. Representative Western blots of the protein levels quantified. For clarity of the results, the representative Western blots are cropped, for raw data see Supplementary Information. All error bars represent ± s.e.m.