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. 2019 Apr 25;9:6568. doi: 10.1038/s41598-019-42793-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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OTU profiles of the samples and phylogenetic analysis of all OTUs. (A) Heat map of OTU profiles (y-axis) inferred from PacBio. The x-axis shows 23 samples. The number of reads per OTU was normalized by total number of reads from the sample. For each sample, the number of reads per OTU represents total number of reads (the number of reads were transformed to log 2) from the sample. The asterisk denoted OTUs show the new OTUs found in this study. (B) The Bayesian unrooted phylogenetic tree of representative sequences OTUs. Red text labels represent the new OTUs. The scale bar at the base indicates number of substitutions per site.