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. 2019 Apr 25;9:6568. doi: 10.1038/s41598-019-42793-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Halomonas appeared in the midgut of MED B. tabaci verified by application of the v4-v5 region of using dissected midguts (A) and fluorescence in situ hybridization (B,C). (D) Phylogenetic tree revealed the relationship of this Halomonas strain with the other published ones. New bacteria-specific probes (green) conjugated to cy5 were used, and the nuclei were stained with DAPI (blue). Portiera is used as a control for Halomonas. Portiera-specific probe (red) is expected not to have occurred in the gut of B. tabaci. B, Halomonas was present in gut of MED; C, Halomonas was not detected in the gut of Asia II 1 due to its low content.