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. 2019 Apr 25;9:6547. doi: 10.1038/s41598-019-43057-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of M1 and M2 markers on peritoneal macrophage. Expression levels of M1 (CD11c (A), iNOS (B) and M2 (CD206 (C), Arg1(D)) markers were analyzed in macrophages from the peritoneal macrophages (C) of T. spiralis-infected or control mice (2 weeks P.I) by using FACS canto II. After staining, the expressions of CD11c, iNOS, CD206 and Arg1 were plotted. After staining, the macrophages were firstly gated with F4/80 -positive cells and the percentage of CD11c, iNOS, CD206 and Arg1 positive cells calculated using FACS analysis. The graphs show the mean of the fluorescence intensity experimental group in the right panel. Statistical analysis was performed with Student’t t-test. (***p < 0.001; n = 3 mice/group; these results are representative of three independent experiments).