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. 2019 Apr 25;9:6523. doi: 10.1038/s41598-019-43002-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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V2R is localized to primary cilia. (a) Confocal images of renal epithelial from pig (LL-CPK1) and dog (IMCD) show localization of V2R (red) at the base of cilia. Acetylated-α-tubulin (acet-α-tub) used as ciliary marker is shown in green and nucleus in blue. Maximum intensity projection images from accumulated z-stack for LL-CPK1 (scrambled and V2R-knockdown), IMCD and endothelial cells are shown in Supplementary Figs S3 and S4. (b) Phase contrast images represent isolated cilia from scrambled control and V2R-knockdown LL-CPK1 cells. (c) Dot blot indicates the presence of V2R in both isolated-cilia and cell-body extracts. Isolated cilia extracts are confirmed by the presence of acetylated-α-tubulin.