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. 2019 Apr 25;9:6523. doi: 10.1038/s41598-019-43002-2

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Forskolin increases cAMP level in cilioplasm and cytoplasm. Representative time-lapse images of 1/cADDis, mCherry channels and Ratio (mCherry/cADDis) with their corresponding line graphs showing cAMP levels in response to forskolin (FSK) in (a) scrambled and (b) V2R-knockdown cells. Color bar on the left indicates cAMP level from low (black) to high (green) for 1/cADDis channel. cAMP level from low (black) to high (yellow) are shown on the right color bar for the ratio images. Corresponding individual raw traces (red) and average signal (blue) are plotted in line graphs for 1/cADDis, mCherry and ratio signals in the cilioplasm and cytoplasm. Arrows indicate stimuli. N = 4 for each group.