Table 1.

Simulated gastrointestinal digestion

Parameter	Compartments
	Stomach	Duodenum	Jejunum	Ileum	Caecum
pH value	2.5	5.5	6.5	7.5	7.5
Incubation time (h)	2	0.25	3	4	1
	Pepsin 3 g/L	Pancreatin 10 g/L; bile salts 3 g/L

Simulated gastrointestinal digestion for unencapsulated and encapsulated yeasts consisted of five compartments with a specific pH value and incubation time for each compartment (for a total of 10.25 h). Pepsin was added in the stomach whereas pancreatin and bile salts were added when simulating the duodenum. Whereas unencapsulated and encapsulated control yeasts were maintained at constant pH 6.5 for 10.25 h (Gbassi et al., 2011)