Table 3.

Identification of differentially accumulated proteins in the probiotic yeast S. boulardii

Banda	Protein	Identifierb	Exp. Mwc	Theor. Mw/pId	Mascot score	PM/SCe
Control conditions
17	10 kDa heat shock protein, mitochondrial	CH10_YEAST	6.4–13.0	11.4/8.96	79	2/25%
15	40S ribosomal protein S12	RS12_YEAST	18.4–20.9	15.5/4.68	66	2/14%
11	40S ribosomal protein S5	RS5_YEAST	28.1–30.7	25.1/8.63	409	3/17%
13	40S ribosomal protein S7-A	RS7A_YEAST	24.2–26.2	21.6/9.83	55	2/13%
9	60S acidic ribosomal protein P0	RLA0_YEAST	34.4–38.2	33.7/4.75	89	4/14%
15	60S ribosomal protein L14-A	RL14A_YEAST	18.4–20.9	15.2/10.94	118	3/26%
13	60S ribosomal protein L18-A	RL18A_YEAST	24.2–26.2	20.6/11.70	72	2/11%
15	60S ribosomal protein L25	RL25_YEAST	18.4–20.9	15.7/10.11	198	2/18%
17	60S ribosomal protein L30	RL30_YEAST	6.4–13.0	11.4/9.80	74	3/45%
16	60S ribosomal protein L31-A	RL31A_YEAST	13.0–18.4	12.9/9.99	47	2/23%
16	60S ribosomal protein L36-A	RL36A_YEAST	13.0–18.4	11.1/11.60	48	2/21%
6	6-Phosphogluconate dehydrogenase, decarboxylating 1	6PGD1_YEAST	45.5–54.2	53.9/6.19	164	4/11%
3	Aconitate hydratase, mitochondrial	ACON_YEAST	79.3–88.1	85.7/8.17	197	8/11%
2	Aminopeptidase 2, mitochondrial	APE2_YEAST	88.1–98.1	108.1/8.11	47	4/5%
11	Heat shock protein 26	HSP26_YEAST	28.1–30.7	23.9/5.31	60	2/13%
4	Heat shock protein SSA2	HSP72_YEAST	64.4–79.3	69.6/4.95	240	11/25%
4	Heat shock protein SSC1, mitochondrial	HSP77_YEAST	64.4–79.3	70.6/5.48	121	8/16%
5	Hexokinase-1	HXKA_YEAST	54.2–64.4	53.9/5.28	142	6/18%
16	Histone H4	H4_YEAST	13.0–18.4	11.36/11.3	61	3/29%
6	Homocysteine/cysteine synthase	CYSD_YEAST	45.5–54.2	48.7/5.97	167	3/7%
4	Isocitrate lyase	ACEA_YEAST	64.4–79.3	62.7/5.97	115	5/10%
7	Malate dehydrogenase, cytoplasmic	MDHC_YEAST	41.2–45.5	41.0/6.41	150	3/16%
7	Mannose-1-phosphate guanylyltransferase	MPG1_YEAST	41.2–45.5	39.7/5.95	154	3/12%
8	Putative pyridoxal reductase	PLR1_YEAST	38.2–41.2	38.9/5.66	104	3/11%
16	Restriction of telomere capping protein 3	SDO1L_YEAST	13.0–18.4	12.0/5.05	108	3/44%
8	Ribonucleoside-diphosphate reductase small chain 2	RIR4_YEAST	38.2–41.2	40.1/5.11	81	4/19%
7	Sphingolipid long chain base-responsive protein PIL1	PIL1_YEAST	41.2–45.5	38.3/4.54	63	4/17%
8	Transaldolase	TAL1_YEAST	38.2–41.2	37.1/6.09	119	4/15%
17	Ubiquitin-60S ribosomal protein L40	RL40A_YEAST	6.4–13.0	14.8/9.87	153	3/26%
Simulated gastrointestinal digestion
7	Dihydrolipoyl dehydrogenase, mitochondrial	DLDH_YEAST	41.2–45.5	54.3/8.07	160	4/12%
10	Glyceraldehyde-3-phosphate dehydrogenase 1	G3P1_YEAST	30.7–34.4	35.8/8.29	138	5/23%
2	Invertase 2	INV2_YEAST	88.1–98.1	60.7/4.61	84	6/11%
8	Ribonucleoside-diphosphate reductase small chain 1	RIR2_YEAST	38.2–41.2	46.2/5.14	115	3/7%
6	S-(hydroxymethyl)glutathione dehydrogenase	FADH_YEAST	45.5–54.2	41.9/6.33	58	3/8%
7	Saccharopepsin	CARP_YEAST	41.2–45.5	44.7/4.70	224	7/20%
6	Serine hydroxymethyltransferase, cytosolic	GLYC_YEAST	45.5–54.2	52.5/6.98	87	4/10%
14	Superoxide dismutase [Cu–Zn]	SODC_YEAST	20.9–24.2	16.0/5.62	207	9/88%
5	Threonine synthase	THRC_YEAST	54.2–64.4	57.6/5.46	190	5/12%
5	Vacuolar aminopeptidase 1	AMPL_YEAST	54.2–64.4	57.3/5.55	181	4/12%
Encapsulated cells
12	40S ribosomal protein S8-A	RS8A_YEAST	26.7–28.1	22.6/10.67	105	3/20%
Unencapsulated cells
3	Acetyl-coenzyme A synthetase 1	ACS1_YEAST	79.3–88.1	74.5/6.15	58	2/3%
10	Succinate-CoA ligase [ADP-forming] subunit alpha, mitochondrial	SUCA_YEAST	30.7–34.4	35.2/8.59	81	3/11%

^aBand numbers correspond to Fig. 2

^bMnemonic identifier according to SwissProt

^cExperimental molecular mass

^dTheoretical molecular mass/isoelectric point

^ePeptides matched/sequence coverage