Figure 14.

Localization of hAChE-S, T40 (A), and β-sheet derived hAChE peptides (B) in the brain of hAChE-S/hAPPswe double transgenic mice. Frozen brain sections (12 μm) from double transgenic mice were double-labeled with the Elite ABC M.O.M. kit or Vectastain Elite ABC rabbit IgG kit using a biotinylated anti-mouse or anti-rabbit secondary antibody and FITC or Texas red–conjugated avidin (Vector Laboratories). The scale bar represents 10 μm. Shown is one of the z slices from a z stack, with the inset showing a z projection. The right panels show quantitation of the percentage of overlap between the two fluorophores within the plaques (e.g. HR2 labeling overlapping with Bam10 labeling), with plaques examined from at least two different sections per conditions (with the order of the antibody/reagents have been switched for the staining). The brains from two different mice were examined. Each value is derived from one individual plaque. Also indicated is the mean, and error bars are S.E.