Figure 2.

A, example of concentration-dependent BiP oligomerization. The BiP monomer elutes at 6.8 min. B, BiP can be maintained in a predominantly monomeric state at concentrations below 100 nm under all nucleotide conditions. Error bars are the S.E. of the mean for at least three measurements. C, design of a previously established BiP FRET pair (25). D, the BiP fluorescence spectrum shows anticorrelated changes at donor and acceptor emission under ADP and ATP conditions. mAU, milliabsorbance units; cps, counts per second.