Figure 3.

Fluorescence depolarization binding measurements show strong binding between BiP and Grp94 under ADP conditions and weak binding under ATP conditions. Error bars are the S.E. of the mean for at least three measurements. Buffer conditions were 25 mm Tris, pH 7.5, 50 mm KCl, 1 mm MgCl₂, 1 mm ADP or ATP, and 1 mg/ml BSA at 37 °C. mP, millipolarization units.