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BiP activates the ATPase of Grp94 ATPase. The ATPase-deficient construct of BiP (T229G) shows that the increase in activity is due to Grp94. The binding-deficient mutants (BiP E243A and Grp94 K467A) show no activation, yielding roughly additive ATPase results. Error bars are the S.E. of the mean for at least three measurements. Buffer conditions were 25 mm Tris, pH 7.5, 50 mm KCl, 1 mm MgCl2, 1 mm ATP, and 1 mg/ml BSA at 37 °C.
