. 2019 Feb 28;294(16):6483–6493. doi: 10.1074/jbc.RA119.007712

Table 2.

Kinetic parameters of the glycan synthesis reaction catalyzed by PapP when 10 mm Glc1P was used as a donor

Kinetic parameters for GH149 enzymes (EgP1 and Pro_7066) reported previously are presented for comparison (24). NA, no activity.

Acceptor	PapP			EgP1			Pro_7066
Acceptor	k_cat	K_m	k_cat/K_m	k_cat	K_m	k_cat/K_m	k_cat (s⁻¹)	K_m	k_cat/K_m
	s⁻¹	mm	s⁻¹ mm⁻¹	s⁻¹	mm	s⁻¹ mm⁻¹	s⁻¹	mm	s⁻¹ mm⁻¹
Glc	NA	NA	NA	1.10 ± 0.03	0.56 ± 0.06	1.99	1.66 ± 0.04	0.29 ± 0.03	5.79
G2	31.6 ± 1.0	1.58 ± 0.17	20.0	1.08 ± 0.03	0.67 ± 0.08	1.62	1.54 ± 0.01	0.25 ± 0.02	6.03
G3	33.3 ± 1.6	1.05 ± 0.19	31.6	1.12 ± 0.02	1.26 ± 0.09	0.89	1.53 ± 0.02	0.37 ± 0.03	4.16
G4	33.3 ± 1.7	1.82 ± 0.29	18.3	1.12 ± 0.03	1.41 ± 0.13	0.79	1.39 ± 0.01	0.36 ± 0.02	3.89
G5	27.4 ± 0.7	1.61 ± 0.13	17.0	1.13 ± 0.03	2.29 ± 0.19	0.50	1.27 ± 0.01	0.32 ± 0.02	4.04
G6	30.0 ± 0.6	2.31 ± 0.56	13.0	1.10 ± 0.03	2.88 ± 0.23	0.38	1.18 ± 0.04	0.26 ± 0.04	4.62