Table 2.

IC₅₀ values of different compounds on the TtCE15A WT enzyme and enzyme variants using BnzGlcA as substrate

NM, not measured; NI, no/minor inhibition detected (>87% retained enzyme activity after maximum addition of inhibitor).

TtCE15A	FA	SA	pCoA	XUXXR
	mm	mm	mm	mm
WT	0.55 ± 0.045	0.56 ± 0.030	0.83 ± 0.04	5.51 ± 0.28
F174A	0.084 ± 0.005	0.19 ± 0.022	4.84 ± 0.47	NM
F174D	0.40 ± 0.040	0.77 ± 0.064	26.0 ± 12.0	NM
W376A	NM	NM	NM	NI