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The inhibition of the NADH oxidase activity in SMPs by S1QEL1.5. Trace a, the NADH oxidase activity without inhibitor (control); trace b, the NADH oxidase activity initiated by adding NADH (200 μm) after 4 min incubation with S1QEL1.5 (50 μm); trace c, S1QEL1.5 (50 μm) was added to SMPs at ∼1 min after initiation of the reaction by adding NADH (200 μm). The final mitochondrial protein concentration was 10 μg/ml.
