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. 2019 Mar 1;294(16):6550–6561. doi: 10.1074/jbc.RA119.007687

Figure 3.

Figure 3.

Time-dependent disappearance of the inhibitory effects of S1QEL1.5 and S1QEL1.5_D1. A, the inhibition of NADH oxidase activity (forward electron transfer) by S1QEL1.5 (5.0 μm, black bars) or S1QEL1.5_D1 (5.0 μm, gray bars) was determined after different incubation periods with SMPs at 30 °C. The averaged control forward electron transfer activity was 580 ± 55 nmol of NADH/min/mg of proteins. The final mitochondrial protein concentration was 30 μg/ml. B, the inhibition of ubiquinol-NAD+ oxidoreductase activity (reverse electron transfer) by S1QEL1.5 (6.3 μm, black bars) or S1QEL1.5_D1 (6.3 μm, gray bars) was determined after different incubation periods with SMPs at 30 °C. The averaged control reverse electron transfer activity was 50 ± 6.0 nmol of NADH/min/mg of proteins. The final mitochondrial protein concentration was 100 μg/ml. Values in graphs are mean ± S.E. (n = 3).