Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Mar 1;294(16):6550–6561. doi: 10.1074/jbc.RA119.007687

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2019 Banba et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

Figure 5. — Effects of S1QELs on alkynylation of 49 kDa-Asp¹⁶⁰ by AL1. A, alkynylation of 49 kDa-Asp¹⁶⁰ in intact complex I was achieved via LDT chemistry using AL1 (0.1 μm). The alkynylation can be visualized by conjugating a fluorescent tag TAMRA-N₃ to [Asp¹⁶⁰(COO)-(CH₂)₂-CCH] via click chemistry after solubilizing SMPs (see “Experimental procedures” and Fig. S2). Approximately 30 μg of proteins were loaded into each well. Upper panel, gel image of SDS-PAGE analysis used for LDT chemistry; lower panel, the extent of suppression by S1QELs (10 μm each, 100-fold of AL1). Values in graphs are mean ± S.E. (n = 3–4). **, p < 0.001 compared with control (one-way ANOVA followed by Dunnet's test). B, suppression of the alkynylation by different concentrations of S1QEL1.1 (5.0–50 μm). Data are representative of three independent experiments.

Inline graphic — Effects of S1QELs on alkynylation of 49 kDa-Asp¹⁶⁰ by AL1. A, alkynylation of 49 kDa-Asp¹⁶⁰ in intact complex I was achieved via LDT chemistry using AL1 (0.1 μm). The alkynylation can be visualized by conjugating a fluorescent tag TAMRA-N₃ to [Asp¹⁶⁰(COO)-(CH₂)₂-CCH] via click chemistry after solubilizing SMPs (see “Experimental procedures” and Fig. S2). Approximately 30 μg of proteins were loaded into each well. Upper panel, gel image of SDS-PAGE analysis used for LDT chemistry; lower panel, the extent of suppression by S1QELs (10 μm each, 100-fold of AL1). Values in graphs are mean ± S.E. (n = 3–4). **, p < 0.001 compared with control (one-way ANOVA followed by Dunnet's test). B, suppression of the alkynylation by different concentrations of S1QEL1.1 (5.0–50 μm). Data are representative of three independent experiments.