Table 1.
Yeast strains used in this study
| Strain | Genotype | Refs. |
|---|---|---|
| SUB62a | MATa lys2-801 leu2-3, 2--112 ura3-52 his3-Δ200 trp1-1 | 57 |
| SP1694B | MATa nas6::TRP1 | 19 |
| sJR192A | MATa rpn14::hphMX | 13 |
| SP1665A | MATa nas6::TRP1 rpn14::hphMX | 19 |
| SP861 | MATa hsm3::KAN | 12 |
| SP1654B | MATa nas2::KAN | 19 |
| SP2497B | MATa nas2::KAN hsm3::KAN | 19 |
| SP2781A | MATα rpt1::Prpt1-yEGFP1F-RPT1-LEU2 | Derived from Ref. 14 |
| SP3033A | MATa rpt1::Prpt1-yEGFP1F-RPT1-LEU2 nas2::KAN | This study |
| SP3032A | MATa rpt1::Prpt1-yEGFP1F-RPT1-LEU2 hsm3::KAN | This study |
| SP3031A | MATα rpt1::Prpt1-yEGFP1F-RPT1-LEU2 nas2::KAN hsm3::KAN | This study |
| SP3024A | MATa rpt1::Prpt1-yEGFP1F-RPT1-LEU2 nas6::HIS3 | This study |
| SP3023A | MATα rpt1::Prpt1-yEGFP1F-RPT1-LEU2 rpn14::hphMX | This study |
| SP3022A | MATa rpt1::Prpt1-yEGFP1F-RPT1-LEU2 nas6::HIS3 rpn14::hphMX | This study |
| SP2017 | MATa rpt6::Prpt6-yEGFP1F-RPT6-LEU2 | Derived from Ref. 14 |
| SP3027A | MATα rpt6::Prpt6-yEGFP1F-RPT6-LEU2 nas2::KAN | This study |
| SP3026A | MATα rpt6::Prpt6-yEGFP1F-RPT6-LEU2 hsm3::KAN | This study |
| SP3025A | MATα rpt6::Prpt6-yEGFP1F-RPT6-LEU2 nas2::KAN hsm3::KAN | This study |
| SP2965C | MATa rpt6::Prpt6-yEGFP1F-RPT6-LEU2 nas6::HIS3 | This study |
| SP2964B | MATα rpt6::Prpt6-yEGFP1F-RPT6-LEU2 rpn14::hphMX | This study |
| SP2963A | MATα rpt6::Prpt6-yEGFP1F-RPT6-LEU2 nas6::HIS3 rpn14::hphMX | This study |
| SP2786A | MATa rpn11::RPN11-GFP (HIS3) | This study |
| SP3321A | MATa rpn11::RPN11-GFP (HIS3) nas6::TRP | This study |
| SP3322A | MATα rpn11::RPN11-GFP (HIS3) rpn14:: hphMX | This study |
| SP3323A | MATα rpn11::RPN11-GFP (HIS3) nas6::TRP rpn14:: hphMX | This study |
| SP3324A | MATa rpn11::RPN11-GFP (HIS3) nas2::NAT | This study |
| SP3325A | MATα rpn11::RPN11-GFP (HIS3) hsm3::KAN | This study |
| SP3326A | MATα rpn11::RPN11-GFP (HIS3) nas2::NAT hsm3::KAN | This study |
| SP3364 | MATα rpt6::Prpt6-yEGFP1F-RPT6-LEU2 hsp42::KAN | This study |
| SP3367 | MATa rpt6::Prpt6-yEGFP1F-RPT6-LEU2 hsp42::KAN nas6::HIS3 rpn14::hphMX | This study |
| SP3368 | MATα rpt1::Prpt1-yEGFP1F-RPT1-LEU2 hsp42::KAN | This study |
| SP3371A | MATα rpt1::Prpt1-yEGFP1F-RPT1-LEU2 hsp42::KAN nas2::KAN hsm3::KAN | This study |
| SP3363A | MATα rpt1::Prpt1-yEGFP1F-RPT1-LEU2 pdr5::KAN | This study |
| SP3359A | MATa rpt1::Prpt1-yEGFP1F-RPT1-LEU2 pdr5::KAN nas2::NAT | This study |
| SP3360A | MATa rpt1::Prpt1-yEGFP1F-RPT1-LEU2 pdr5::KAN hsm3::KAN | This study |
| SP3358A | MATa rpt1::Prpt1-yEGFP1F-RPT1-LEU2 pdr5::KAN nas2::NAT hsm3::KAN | This study |
| SP3065A | MATα rpt6::Prpt6-yEGFP1F-RPT6-LEU2 pdr5::KAN | This study |
| SP3067B | MATa rpt6::Prpt6-yEGFP1F-RPT6-LEU2 pdr5::KAN nas6::HIS3 | This study |
| SP3066B | MATa rpt6::Prpt6-yEGFP1F-RPT6-LEU2 pdr5::KAN rpn14::hphMX | This study |
| SP3064C | MATa rpt6::Prpt6-yEGFP1F-RPT6-LEU2 pdr5::KAN nas6::HIS3 rpn14::hphMX | This study |
a All strains are isogenic to sub62 genetic background.