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A, band-shift assay of 1 μm Cy5–DNA incubated with 50 μm TNFα. B, DNA binding to TNFα measured by MTS, where the concentrations of Cy5–dsDNA and Cy5–Y-DNA are 10 nm, and TNFα concentration ranged from 122.5 nm to 250 μm. C, CD spectra of TNFα (10 μm) in the presence or absence of DNA (10 μm) after subtracting the DNA spectra.
