Figure 5.

Effects of S78A and S78D mutations on CEP131 interaction with CEP152. A, HeLa cells were simultaneously transfected with CEP131 siRNA and the indicated GFP-tagged CEP131 construct. Cells were then fixed and stained for CEP152. The amount of CEP152 at the centrosome was quantified and is shown in the adjacent dot plot. Insets in the CEP152 images show magnified CEP152 at the centrosome. B, WT HeLa cells and CEP131 knockout cell lines (KO4E and KO9) were stained for CEP152, and the amount of CEP152 at the centrosome was quantified. Insets, magnifications of the centrosome. Each dot represents a single cell. Bars, means ± S.D. (error bars). Scale bars, 10 μm. ***, p < 0.0001; ns, not significant.