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. 2019 Feb 25;294(16):6531–6549. doi: 10.1074/jbc.RA118.004867

Figure 6.

Figure 6.

Chronic CEP131 depletion causes dispersion of centriolar satellites and is rescued by CEP131 phosphorylation. A, CEP131 Western blotting in WT HeLa cells compared with two CEP131 knockout cell lines, KO4E and KO9. B, immunofluorescent images showing loss of CEP131 in the knockout cell lines. C, immunofluorescent imaging of centrioles and centriolar satellites in the knockout cell lines. D, quantification of centriolar satellites in WT HeLa cells and the two CEP131 knockout lines. E, quantification of centrioles (centrin foci) in WT and CEP131 knockout HeLa lines. F, representative images of primary cilia in WT and CEP131 knockout HeLa lines. G, quantification of primary cilia in WT HeLa cells and the two CEP131 knockout lines. Scale bars, 10 μm. H, cell counts over 12 days in WT HeLa cells compared with two CEP131 knockout cell lines. I and J, proliferation in cells treated with serum-free medium (I) or with 1% serum (J). Proliferation was normalized to untreated cells within each cell line. K, representative images of centriolar satellites (marked by PCM1) in CEP131 knockout cells transfected with either WT, S78A, or S78D CEP131 transgenes. L, quantification of the percentage of cells with dispersed and aggregated PCM1. M, quantification of the percentage of PCM1 intensity within a 2.5-μm radius of the center of the centrosome. Nocodazole is used as a positive control for centriolar satellite dispersion. All experiments in this figure utilize HeLa cells. Bars, means ± S.E. (error bars). Scale bars, 10 μm. *, p < 0.05; ns, not significant.