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. 2019 Feb 25;294(16):6531–6549. doi: 10.1074/jbc.RA118.004867

Figure 7.

Figure 7.

CEP72 staining recapitulates regulation of centriolar satellite integrity by CEP131 Ser-78 phosphorylation status. A, to ensure that our results regarding PLK4 phosphorylation of CEP131 regulating centriolar satellite integrity were not specific to PCM1, we also analyzed another marker of centriolar satellites, CEP72. WT or CEP131 KO HeLa cell lines were transfected with GFP-tagged CEP131 constructs (WT, S78A, and S78D) versus GFP-only control. Nocodazole treatment served as a positive control for satellite dispersion. Representative images are shown. B, CEP72 was assessed as either dispersed or aggregated, just as done for PCM1 staining of centriolar satellites in other figures. All experiments in this figure utilize HeLa cells. DAPI, 4′,6-diamidino-2-phenylindole. Bars, means ± S.D. (error bars) from three replicates. Scale bars, 10 μm. At least 300 cells were analyzed for each condition. *, p < 0.05.