Figure 8.

Dynein is required for satellite aggregation with CEP131 S78D phosphomimetic. A, representative images of HeLa cells treated with nocodazole and the dynein inhibitor ciliobrevin D. B, quantification of centriolar satellites (PCM1 staining) in the conditions shown in A. C, representative images of WT HeLa cells transfected with CEP131 S78D and treated with ciliobrevin D. D, quantification of centriolar satellites (PCM1 staining) in the conditions shown in C. Bars, means ± S.D. (error bars) from three replicates. Scale bars, 10 μm. At least 300 cells were analyzed for each condition. *, p < 0.05.