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. 2019 Feb 25;294(16):6531–6549. doi: 10.1074/jbc.RA118.004867

Figure 9.

Figure 9.

PLK4 phosphorylation of CEP131 at Ser-78 is critical for proper centriolar satellite integrity. A, WT HeLa cells were transfected with GFP or GFP-tagged WT CEP131 or S78A or S78D mutant. Western blotting demonstrating transfection efficiency. B, images of centriolar satellites in these transfected cells after treatment with the PLK4 inhibitor centrinone B. Scale bar, 10 μm. C, qualitative assessment of centriolar satellites as either dispersed or aggregated. D, quantitative assessment of centriolar satellite dispersion/aggregation. All experiments in this figure utilize WT HeLa cells. Bars, means ± S.E. (error bars). Scale bars, 10 μm. *, p < 0.05; **, p < 0.01; ***, p < 0.0001; ns, not significant.