Figure 6.

RavD prevents accumulation of LAMP-1 on Legionella-containing vacuoles. A, RAW264.7 macrophages were infected with WT, ΔdotA, ΔravD, or ΔravD+pHA-RavD for 1, 3, 5, and 10 h, and the percentage of LAMP-1–positive vacuoles for each condition was determined. The bar graph displays the average and standard deviation for the percentage of LCVs that were LAMP-1–positive at each time point. Data were obtained from three independent experiments where at least 100 different vacuoles were assessed. Asterisks denote data that showed a significant difference from the control (WT) in an unpaired Student's t test (***, p ≤ 0.001; **, p ≤ 0.01). B, representative confocal microscopy images of LAMP1-stained cells infected with the indicated strains. Scale bars = 2 μm (insets, 0.5 μm).