Subject area:	Immunology and Microbiology
More specific subject area:	HIV-1 Envelope
Method name:	Identifying cleaved, functional HIV-1 Envs
Name and reference of original method:	Adapted from: •Pancera M, Wyatt R (2005); Virology 332:145-156. •Li M, Gao F, Mascola JR et al (2005); J Virology 79(16):10108-10125. •Ringe R, Thakar M, Bhattacharya J. (2010); Retrovirology 7: 76. •Chakrabarti BK, Pancera M, Phogat S, et al (2011); AIDS Res Hum Retroviruses 27(8):877-87. •Boliar S, Das S, Bansal M, Shukla BN, Patil S, et al (2015); PLoS One 10: e0122443.
Resource availability:	•R-Phycoerythrin-conjugated AffinityPure F(ab’)2 Fragment Goat Anti-Human IgG, F(ab’)2 Fragment Specific (minimal cross-reaction to Bovine, Horse, and Mouse Serum Proteins); Cat# 109-116-097; Jackson ImmunoResearch Laboratories Inc. •FlowJo software, version 10.0.6, Tree Star Inc. •sCD4-183, Cat# 7356; sCD4, Cat# 4615; NIH AIDS Reagent Program •Dounce homogenizer; Cat# D8938; Sigma-Aldrich •Plasma membrane protein extraction kit; Cat# ab65400; Abcam •EZ-link Sulfo-NHS-LC-Biotin; Cat# 21335; Thermo Scientific •Neutravidin agarose; Cat#. 29204; Thermo Scientific •anti-gp120 Env (clade A, B, C) antibody; Cat# 5419, 5414, 5411; AbLinc.