Table 1.
Identification of exosome—associated proteins.
| Accession | Peptides | Score | ANOVA (p) | Fold | log 2 (fold) | Description/molecular function | Average normalised abundances | Localization | |
|---|---|---|---|---|---|---|---|---|---|
| Mock | siCA1 | ||||||||
| Binding | |||||||||
| P63104 | 5 (4) | 43.73 | 6.32E−03 | 2.08 | 1.06 | 14‐3‐3 protein zeta/delta OS = Homo sapiens GN = YWHAZ PE = 1 SV = 1 | 532.14 | 1104.66 | Cytoplasm |
| P43686 | 4 (4) | 33.23 | 7.16E−03 | 2.16 | 1.11 | 26S protease regulatory subunit 6B OS = Homo sapiens GN = PSMC4 PE = 1 SV = 2 | 367.91 | 796.05 | Nucleus |
| P35998 | 2 (2) | 12.88 | 1.88E−03 | 1.99 | 0.99 | 26S protease regulatory subunit 7 OS = Homo sapiens GN = PSMC2 PE = 1 SV = 3 | 437.08 | 869.42 | Cytoplasm |
| P07355 | 7 (6) | 70.95 | 3.01E−03 | 2.13 | 1.09 | Annexin A2 OS = Homo sapiens GN = ANXA2 PE = 1 SV = 2 | 1131.16 | 2409.8 | Extracellular matrix, secretion |
| P01024 | 2 (2) | 19.10 | 0.01 | 3.09 | 1.63 | Complement C3 OS = Homo sapiens GN = C3 PE = 1 SV = 2 | 457.65 | 1414.35 | Extracellular matrix, secretion |
| P62807 | 7 (6) | 67.80 | 0.03 | 1.90 | 0.93 | Histone H2B OS = Homo sapiens GN = HIST1H2BF PE = 2 SV = 1 | 2365.09 | 4503.56 | Nucleus |
| P62805 | 5 (5) | 53.81 | 0.04 | 3.31 | 1.73 | Histone H4 OS = Homo sapiens GN = HIST1H4A PE = 1 SV = 2 | 578.73 | 174.87 | Nucleus |
| P0DMV9 | 7 (3) | 61.09 | 0.04 | 1.86 | 0.90 | Heat shock 70 kDa protein 1B OS = Homo sapiens GN = HSPA1B PE = 1 SV = 1 | 391.3 | 726.97 | Cytoskeletion, centrosome |
| P04792 | 5 (4) | 55.78 | 0.03 | 1.85 | 0.89 | Heat shock protein beta‐1 OS = Homo sapiens GN = HSPB1 PE = 1 SV = 2 | 660.19 | 1220.52 | Nucleus, cytoplasm |
| P31943 | 2 (2) | 13.70 | 0.03 | 1.93 | 0.95 | Heterogeneous nuclear ribonucleoprotein H OS = Homo sapiens GN = HNRNPH1 PE = 1 SV = 1 | 161.52 | 312.05 | Nucleus |
| Q14974 | 6 (6) | 48.45 | 0.04 | 1.58 | 0.66 | Importin subunit beta‐1 OS = Homo sapiens GN = KPNB1 PE = 1 SV = 2 | 618.02 | 979.23 | Nucleus, cytoplasm |
| P01130 | 4 (4) | 33.29 | 3.17E−03 | 2.74 | 1.45 | Low‐density lipoprotein receptor OS = Homo sapiens GN = LDLR PE = 1 SV = 1 | 243.49 | 667.06 | Membrane, endosomes, GA |
| P35579 | 6 (6) | 45.79 | 1.31E−06 | 3.24 | 1.70 | Myosin‐9 OS = Homo sapiens GN = MYH9 PE = 1 SV = 4 | 303.04 | 981.28 | Cytoskeletion |
| Q15149 | 2 (2) | 18.81 | 4.58E−03 | 2.61 | 1.38 | Plectin (Fragment) OS = Homo sapiens GN = PLEC PE = 1 SV = 1 | 252.03 | 657.22 | Cytoskeletion |
| P09874 | 2 (2) | 18.84 | 0.01 | 2.84 | 1.51 | Poly [ADP‐ribose] polymerase 1 OS = Homo sapiens GN = PARP1 PE = 1 SV = 4 | 105.09 | 298.46 | Nucleus |
| Q16799 | 2 (1) | 24.71 | 1.30E−05 | 2.77 | 1.47 | Reticulon OS = Homo sapiens GN = RTN1 PE = 1 SV = 1 | 286.49 | 794.43 | Endoplasmatic reticulum |
| P62140 | 2 (2) | 20.67 | 2.14E−05 | 5.00 | 2.32 | Serine/threonine‐protein phosphatase PP1‐beta catalytic subunit OS = Homo sapiens GN = PPP1CB PE = 1 SV = 3 | 299.16 | 1495.44 | Nucleus |
| P48643 | 8 (6) | 73.19 | 3.41E−03 | 1.61 | 0.69 | T‐complex protein 1 subunit epsilon OS = Homo sapiens GN = CCT5 PE = 1 SV = 1 | 1366.32 | 2204.6 | Cytoskeletion |
| Q99832 | 7 (6) | 68.33 | 5.85E−03 | 2.29 | 1.20 | T‐complex protein 1 subunit eta OS = Homo sapiens GN = CCT7 PE = 1 SV = 2 | 864.32 | 1975.35 | Cytoplasm |
| P78371 | 7 (6) | 61.93 | 0.04 | 1.50 | 0.58 | T‐complex protein 1 subunit beta OS = Homo sapiens GN = CCT2 PE = 1 SV = 4 | 908.71 | 1366.06 | Cytoplasm |
| Q9BUF5 | 9 (2) | 66.65 | 6.31E−03 | 3.63 | 1.86 | Tubulin beta‐6 chain OS = Homo sapiens GN = TUBB6 PE = 1 SV = 1 | 47.35 | 171.88 | Cytoskeletion |
| P07996 | 2 (2) | 13.57 | 1.27E−04 | 2.53 | 1.34 | Thrombospondin‐1 OS = Homo sapiens GN = THBS1 PE = 1 SV = 2 | 298.54 | 755.51 | Endoplasmatic reticulum extracellular matrix |
| P13010 | 11 (11) | 99.67 | 4.46E−03 | 1.95 | 0.96 | X‐ray repair cross‐complementing protein 5 OS = Homo sapiens GN = XRCC5 PE = 1 SV = 3 | 1029.18 | 2002.42 | Nucleus |
| Q9Y2P7 | 2 (1) | 18.82 | 1.41E−04 | 3.75 | 1.91 | Zinc finger protein 256 OS = Homo sapiens GN = ZNF256 PE = 4 SV = 1 | 19.27 | 72.33 | Nucleus |
| Catalytic activity | |||||||||
| P43686 | 4 (4) | 33.23 | 7.16E−03 | 2.16 | 1.11 | 26S protease regulatory subunit 6B OS = Homo sapiens GN = PSMC4 PE = 1 SV = 2 | 367.91 | 796.05 | Nucleus |
| P35998 | 2 (2) | 12.88 | 1.88E−03 | 1.99 | 0.99 | 26S protease regulatory subunit 7 OS = Homo sapiens GN = PSMC2 PE = 1 SV = 3 | 437.08 | 869.42 | Cytoplasm |
| P01024 | 2 (2) | 19.10 | 0.01 | 3.09 | 1.63 | Complement C3 OS = Homo sapiens GN = C3 PE = 1 SV = 2 | 457.65 | 1414.35 | Extracellular matrix, secretion |
| P11586 | 2 (2) | 18.33 | 3.43E−03 | 2.41 | 1.27 | C‐1‐tetrahydrofolate synthase_ cytoplasmic OS = Homo sapiens GN = MTHFD1 PE = 1 SV = 1 | 119.51 | 288.08 | Cytoplasm |
| P26641 | 7 (6) | 58.66 | 1.56E−04 | 1.98 | 0.99 | Elongation factor 1‐gamma OS = Homo sapiens GN = EEF1G PE = 1 SV = 3 | 559.55 | 1108.5 | Cytoplasm |
| P0DMV9 | 7 (3) | 61.09 | 0.04 | 1.86 | 0.90 | Heat shock 70 kDa protein 1B OS = Homo sapiens GN = HSPA1B PE = 1 SV = 1 | 391.3 | 726.97 | Cytoskeletion, centrosome |
| P04792 | 5 (4) | 55.78 | 0.03 | 1.85 | 0.89 | Heat shock protein beta‐1 OS = Homo sapiens GN = HSPB1 PE = 1 SV = 2 | 660.19 | 1220.52 | Nucleus, cytoplasm |
| P35579 | 6 (6) | 45.79 | 1.31E−06 | 3.24 | 1.70 | Myosin‐9 OS = Homo sapiens GN = MYH9 PE = 1 SV = 4 | 303.04 | 981.28 | Cytoskeletion |
| Q14697 | 4 (4) | 34.09 | 0.01 | 2.57 | 1.36 | Neutral alpha‐glucosidase AB OS = Homo sapiens GN = GANAB PE = 1 SV = 1 | 194.31 | 499.1 | Endoplasmatic reticulum, Golgi aparatus |
| P14618 | 22 (20) | 224.48 | 2.91E−06 | 1.99 | 0.99 | Pyruvate kinase PKM OS = Homo sapiens GN = PKM PE = 1 SV = 4 | 3161.71 | 6281.27 | Cytoplasm |
| Q92626 | 12 (12) | 101.96 | 2.13E−04 | 2.07 | 1.05 | Peroxidasin homolog OS = Homo sapiens GN = PXDN PE = 1 SV = 2 | 1346.04 | 2780.28 | Extracelular matrix, secretion |
| O14818 | 7 (6) | 71.05 | 4.55E−03 | 2.46 | 1.30 | Proteasome subunit alpha type‐7 OS = Homo sapiens GN = PSMA7 PE = 1 SV = 1 | 887.97 | 2180.2 | Nucleus |
| P25786 | 5 (5) | 54.34 | 2.06E−03 | 1.97 | 0.98 | Proteasome subunit alpha type‐1 OS = Homo sapiens GN = PSMA1 PE = 1 SV = 1 | 1220.78 | 2401.13 | Nucleus |
| P21980 | 3 (3) | 19.12 | 5.80E−06 | 3.60 | 1.85 | Protein‐glutamine gamma‐glutamyltransferase 2 OS = Homo sapiens GN = TGM2 PE = 1 SV = 2 | 194.67 | 700.1 | Mitochondrion, cytoplasm |
| P62140 | 2 (2) | 20.67 | 2.14E−05 | 5.00 | 2.32 | Serine/threonine‐protein phosphatase PP1‐beta catalytic subunit OS = Homo sapiens GN = PPP1CB PE = 1 SV = 3 | 299.16 | 1495.44 | Nucleus |
| P26639 | 2 (2) | 20.76 | 1.93E−03 | 2.96 | 1.57 | Threonine—tRNA ligase cytoplasmic OS = Homo sapiens GN = TARS PE = 1 SV = 3 | 263.88 | 781.32 | Cytoplasm |
| P60174 | 2 (2) | 16.70 | 0.03 | 14.94 | 3.90 | Triosephosphate isomerase OS = Homo sapiens GN = TPI1 PE = 1 SV = 3 | 24.27 | 362.52 | Cytoplasm |
| Structural molecule activity | |||||||||
| P08865 | 9 (8) | 78.96 | 2.50E−03 | 1.91 | 0.93 | 40S ribosomal protein SA OS = Homo sapiens GN = RPSA PE = 1 SV = 1 | 1045.46 | 1995.95 | Nucleus |
| P68032 | 11 (4) | 110.00 | 5.36E−03 | 2.78 | 1.48 | Actin_ alpha cardiac muscle 1 OS = Homo sapiens GN = ACTC1 PE = 1 SV = 1 | 584.62 | 1626.59 | Cytoskeletion |
| P31943 | 2 (2) | 13.70 | 0.03 | 1.93 | 0.95 | Heterogeneous nuclear ribonucleoprotein H OS = Homo sapiens GN = HNRNPH1 PE = 1 SV = 1 | 161.52 | 312.05 | Nucleus |
| Q15149 | 2 (2) | 18.81 | 4.58E−03 | 2.61 | 1.38 | Plectin (Fragment) OS = Homo sapiens GN = PLEC PE = 1 SV = 1 | 252.03 | 657.22 | Cytoskeletion |
| Q9BUF5 | 9 (2) | 66.65 | 6.31E−03 | 3.63 | 1.86 | Tubulin beta‐6 chain OS = Homo sapiens GN = TUBB6 PE = 1 SV = 1 | 47.35 | 171.88 | Cytoskeletion |
| Translation regulator activity | |||||||||
| P13639 | 18 (17) | 191.62 | 5.30E−03 | 2.74 | 1.45 | Elongation factor 2 OS = Homo sapiens GN = EEF2 PE = 1 SV = 4 | 3820.7 | 1.05E+04 | Nucleus |
| Protein‐protein interaction | |||||||||
| Q6S8J3 | 13 (3) | 138.63 | 1.01E−06 | 2.91 | 1.54 | POTE ankyrin domain family member E OS = Homo sapiens GN = POTEE PE = 1 SV = 3 | 142.49 | 414.62 | Extracellular matrix, secretion |
| Response to stress | |||||||||
| P14625 | 13 (12) | 129.22 | 0.02 | 1.72 | 0.78 | Endoplasmin OS = Homo sapiens GN = HSP90B1 PE = 1 SV = 1 | 1779.22 | 3061.84 | Endoplasmatic reticulum |
| Antioxidant | |||||||||
| Q92626 | 12 (12) | 101.96 | 2.13E−04 | 2.07 | 1.05 | Peroxidasin homolog OS = Homo sapiens GN = PXDN PE = 1 SV = 2 | 1346.04 | 2780.28 | Extracelular matrix, secretion |
| Transporter activity | |||||||||
| Q14974 | 6 (6) | 48.45 | 0.04 | 1.58 | 0.66 | Importin subunit beta‐1 OS = Homo sapiens GN = KPNB1 PE = 1 SV = 2 | 618.02 | 979.23 | Nucleus, cytoplasm |
| Extracelular matrix structural costituent | |||||||||
| P24821 | 5 (4) | 46.13 | 2.93E−03 | 3.4 | 1.77 | Tenascin OS = Homo sapiens GN = TNC PE = 1 SV = 3 | 190.92 | 649.52 | Extracellular matrix, secretion |
| Receptor activity | |||||||||
| P01130 | 4 (4) | 33.29 | 3.17E−03 | 2.74 | 1.45 | Low‐density lipoprotein receptor OS = Homo sapiens GN = LDLR PE = 1 SV = 1 | 243.49 | 667.06 | Membrane, endosomes, GA |
Proteomic profiles from PC3 siCA1 and control PC3 siMock derived exosomes were compared. Proteins with more than 2 matching peptides, statistically significant (p < 0.05) and with more than 1.5‐fold expression (and log2 transformed fold change), were further functionally categorized and listed in this table.