Figure 5.

Glucose‐induced chemerin/ChemR23 could cause injury and inflammation in GEnCs. A, Representative Western blot images and summarized data showed changes of chemerin, ChemR23, CD31 and TGF‐β1 in cultured GEnCs stimulated by HG at concentration of 40 mmol/L for 12 h or 24 h. Mannitol was used as osmolality control. B, Total RNA was extracted and qRT‐PCR was used to measure the expression of mRNA of each gene in HG‐stimulated GEnCs. GAPDH was used as internal control. C, Relative concentrations of inflammatory factors in the supernatant of HG‐stimulated GEnCs (normalized to control). D, Chemerin was added into cultural medium as a stimulator at final concentration of 10 or 20 nmol/L for 24 h. Total protein was extracted and Western blot was used to show the changes of CD31 and TGF‐β1. E, qRT‐PCR showed the expression of CD31 and TGF‐β1 in chemerin‐stimulated GEnCs. F, GEnCs were stimulated by chemerin for 24 h before the supernatant was collected. ELISA was used to detect the concentrations of inflammatory factors. For all experiments, *P < 0.05 vs control (n = 3). HG, high glucose, M, mol/L