Figure 3.

The overexpression of β‐arrestin2 (ARRB2) suppressed the NF‐κB/COX‐2/MMP‐9 signalling pathway activated by MDP stimulation. BV2 cells were transfected with either ARRB2 full‐length vector or control vector. Western blot were used to analyse for the protein levels of NF‐κB p‐P65 (A), IκBα (B) and COX‐2 (C) in BV2 cells after treated with 2 μg/mL MDP for 12 h. The activity of MMP‐9 (D) was analysed by Gelatin Zymography. Data are means ± SEM from three independent experiments. *P < 0.05 compared with indicated groups