Figure 6.

β‐arrestin2 (ARRB2) deficiency exacerbated stroke outcomes induced by NOD2 stimulation after cerebral ischaemia‐reperfusion injury. Wild type (WT) and β‐arrestin 2 deficient (ARRB2^−/−) mice were subjected to 2 h MCAO and 24 h reperfusion. MDP, an extrinsic ligand of NOD2 was intraventricularly administered to mice 30 min before MCAO. (A) Neurological deficit scores (B) Representative photographs of coronal brain sections following infarction, stained with 2, 3, 5‐triphenyltetrazolium chloride (TTC). (C) Summary of cerebral infarct volume in brains. The infarct volume was expressed as the percentage of the contralateral hemispheric area. Western blot analysis of NF‐κB p‐P65 (D), IκBα (E) and COX‐2 (F) in the penumbral cortex. Results are representative of eight independent experiments. *P < 0.05 compared with indicated groups