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. 2019 Mar 18;23(5):3166–3177. doi: 10.1111/jcmm.14171

Figure 3.

Figure 3

Validation on relationship among CDR1as, miR‐7 and CCNE1 showed that CDR1as competitively inhibits miR‐7 to regulate CCNE1. (A) TargetScan was utilized to predict the bind site of CDR1as and miR‐7; (B), RNA Binding Protein Immunoprecipitation Assay was applied to detect the bind of CDR1as and miR‐7; (C), RT‐PCR was used to determine the expression of CDR1as; (D), TargetScan predicted that CCNE1 was a target gene of miR‐7; (E), Double luciferase reporter assay confirmed that CCNE1 is a target gene of miR‐7; (F), Western blot of CCNE1 on MCF‐7/5‐fluorouracil (5‐FU) and MDA‐MB‐231/5‐FU cells in each group; (G), expression of CCNE1 in MCF‐7/5‐FU and MDA‐MB‐231/5‐FU cells in each group; *, compared with other groups, P < 0.05; #, comparison among groups, P < 0.05