Figure 5.

Combined treatment with Polydatin (PD) and 2‐deoxy‐D‐glucose (2‐DG) inhibits the glycolytic phenotype in breast cancer cells. (A and B) The protein expression levels of GLUT1, LDH‐A, HK2 and HIF‐1α were detected using western blots. β‐actin was used as an internal control. (C and E) The protein expression levels of HK2 in si‐HK2 and si‐NC transfected 4T1 and MCF‐7 cells were determined using Western blot. β‐actin was used as an internal control. (F) Cell counting Kit‐8 kits were used to assess the proliferation of 4T1 and MCF‐7 cells after transfected with siHK2 and combination treatment with PD and 2‐DG at 0 h, 12 h, 24 h, 48 h and 72 h. The symbols * and ** denote significant differences of P < 0.05 and P < 0.01, respectively