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. 2019 Feb 7;23(5):3257–3270. doi: 10.1111/jcmm.14212

Figure 5.

Figure 5

AR is a target gene of miR‐205. A, binding regions between AR 3'‐UTR and miR‐205 sequence; B, luciferase activity of the AR Wt and AR Mut after transfection; C, RNA pull‐down results showed that AR binds to miR‐205; D, the expression of miR‐205 and AR in cells treated with miR‐205 mimic, miR‐205 inhibitor, AR overexpression, and miR‐205 mimic +AR overexpression determined by qRT‐PCR; E, the grey value of miR‐205 and AR protein bands in cells treated with miR‐205 mimic, miR‐205 inhibitor, AR overexpression and miR‐205 mimic +AR overexpression determined by Western blot analysis; F, the protein levels of miR‐205 and AR in cells treated with miR‐205 mimic, miR‐205 inhibitor, AR overexpression, and miR‐205 mimic +AR overexpression determined by Western blot analysis; *, P < 0.05 vs the NC mimic group or the Bio‐probe NC group or the control and NC groups; measurement data were expressed as mean ± SD; data in each group were analysed with ANOVA; the experiment was repeated three times; DMED, erectile dysfunction rats with diabetic mellitus; miR‐205, microRNA‐205; AR, androgen receptor; NC, negative control; 3'‐UTR, 3'‐untranslated region; qRT‐PCR, quantitative real‐time polymerase chain reaction.