Figure 6.

Sal B induced TAZ up‐expression was mediated by the MEK‐ERK signalling pathway during osteogenic differentiation in MC3T3‐E1 cells. (A) Real‐time RT‐PCR and (B) Western blotting analysis for the relative TAZ expression in Day 3 after the treatment. (C) Bar graphs showed the means ± SD from three independent experiments of the relative TAZ protein levels to GAPDH. (D, E) Western blotting showed that Sal B significantly lifted the p‐ERK/ERK ratio and U0126 blocked this regulation in Day 3 after the treatment. (F, G) The AR‐S quantification confirmed the involvement of MEK‐ERK signalling pathway in the facilitation of the osteogenic differentiation induced by Sal B in Day 14. (n = 3) *P < 0.05 vs the control group (cells cultured in osteogenic medium); ^# P < 0.05 vs the Sal B administration group