Figure 4.

MARCH1 knockdown inhibited human HCC cell migration and invasion through down‐regulated PI3K/P‐AKT/β‐catenin pathways and induced apoptosis. A and B, In vitro migration and invasion assay for the negative control and MARCH1 siRNA in the HepG2 and Hep3B cells. Knockdown of the MARCH1 protein with siRNAs in the human HepG2 and Hep3B HCC cells. C and D, In vitro migration and invasion assay for the HepG2 and Hep3B cells with THP in concentrations of 0, 0.5 and 1.0 μg/mL. E and F, Protein expression of MARCH1, PI3K, AKT, P‐AKT, β‐catenin, MCL‐1, BCL‐2, Cleaved caspase‐3 and Cleaved caspase‐7 in the HepG2 and Hep3B cells. G and H, And in the HepG2 and Hep3B cells with 0, 0.5 and 1.0 μg/mL THP concentrations. All the data in this figure are represented as mean ± SD. **P < 0.01