Skip to main content
. 2019 Feb 22;23(5):3386–3401. doi: 10.1111/jcmm.14235

Figure 5.

Figure 5

MARCH1 overexpression accelerated human HCC progression of proliferation, colony formation, wound healing, transwell migration and invasion by activating the PI3K/P‐AKT/β‐catenin pathways. A, Overexpression of the MARCH1 protein with empty vectors and plasmids in the human HepG2 and Hep3B HCC cells. In vitro (B) cell proliferation and (C) colony formation in the HepG2 and Hep3B cells with empty vectors and MARCH1 overexpression. D, In vitro wound healing assay in the HepG2 and Hep3B cells with empty vectors and MARCH1 overexpression. 40 × images show the wound size at 0, 6, 12 and 48 h after the scratch. E and F, In vitro transwell migration and invasion in the HepG2 and Hep3B cells with empty vectors and MARCH1 overexpression. G, Protein expression of MARCH1, PI3K, AKT, P‐AKT, β‐catenin, MCL‐1, BCL‐2 in the human HepG2 and Hep3B cells transfected with empty vectors and plasmids. All the data in this figure are represented as mean ± SD. *P < 0.05, **P < 0.01