Supplementary Figure 3.

(A) Inhibition of PERK and IRE1α signaling enhance sensitivity to ER stress induced cell death. U2OS and 143b cells were treated with increasing concentration of Tm (0,1,2,5 and 10μM) for the indicated times and percent cell viability was measured using Cell-Titer Glo as described in methods. (B) Measurement of cisplatin mediated caspase 3/7 activation using Caspase-Glo 3/7 assay system (Promega Inc) in OS cells following inhibition of PERK and IRE1α signaling. (C) hFOB cells were treated with or without cisplatin or irinotecan and GSK or STF. Twenty-four hours post-treatment, cell viability was measured as described in methods. In all experiments data were normalized to an untreated control cells and graphed. Data represents mean ± S.E of three experiments.