Figure 4.

Nrf2 regulated Notch signalling in breast cancer cells. A, Expression of Notch1‐4, Dll1, Dll3, Dll4, Jagged1 and Jagged2 mRNA in MCF‐7 and MBA‐DA‐231 cells was examined by qRT‐PCR. GADPH was used as a reference for RNA. B, Expression of Notch1‐4, Dll1, Dll3, Dll4, Jagged1 and Jagged2 protein in MCF‐7 and MBA‐DA‐231 cells was performed by western blotting analysis. The signal obtained with a β‐actin antibody served as loading control. Blots were the representative of three independent experiments. C, Quantitative analysis of Notch1‐4, Dll1, Dll3, Dll4, Jagged1 and Jagged2 protein in MCF‐7 and MBA‐DA‐231 cells was shown. Results were means ± SEM of three independent experiments. **P < 0.01 compared to untreated cells