Figure 5.

Nrf2 regulated Notch signalling via glucose‐6‐phosphate dehydrogenase (G6PD) in breast cancer cells. A, Relative luciferase activity was assayed in MCF‐7 and MBA‐DA‐231 cells. B, Expression of G6PD and Notch1 mRNA in MCF‐7 and MBA‐DA‐231 cells was examined by qRT‐PCR. GADPH was used as a reference for RNA. C, Expression of G6PD and Notch1 protein in MCF‐7 and MBA‐DA‐231 cells was performed by western blotting analysis. The signal obtained with a β‐actin antibody served as loading control. Blots were the representative of three independent experiments. D, Quantitative analysis of G6PD and Notch1 protein in MCF‐7 and MBA‐DA‐231 cells was shown. Results were means ± SEM of three independent experiments. **P < 0.01 compared to untreated cells