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. 2019 Feb 26;23(5):3451–3463. doi: 10.1111/jcmm.14241

Figure 6.

Figure 6

Nrf2 regulated Notch signalling via HIF‐1α in breast cancer cells. A, Expression of HIF‐1α and Notch1 mRNA in MCF‐7 and MBA‐DA‐231 cells was examined by qRT‐PCR. GADPH was used as a reference for RNA. B, Expression of HIF‐1α and Notch1 protein in MCF‐7 and MBA‐DA‐231 cells was performed by western blotting analysis. The signal obtained with a β‐actin antibody served as loading control. Blots were the representative of three independent experiments. C, Quantitative analysis of HIF‐1α and Notch1 protein in MCF‐7 and MBA‐DA‐231 cells was shown. D, Expression of HIF‐1α and Notch1 mRNA in Nrf2 overexpression or knockdown MCF‐7 and MBA‐DA‐231 cells was examined by qRT‐PCR. GADPH was used as a reference for RNA. E, Expression of HIF‐1α and Notch1 protein in Nrf2 overexpression or knockdown MCF‐7 and MBA‐DA‐231 cells was performed by western blotting analysis. The signal obtained with a β‐actin antibody served as loading control. Blots were the representative of three independent experiments. F, Quantitative analysis of HIF‐1α and Notch1 protein in Nrf2 overexpression or knockdown MCF‐7 and MBA‐DA‐231 cells was shown. Results were means ± SEM of three independent experiments. **P < 0.01 compared to untreated cells