Figure 6.

GW9662 inhibited BCa growth in vivo. A, BALB/c nude mice were subcutaneously injected with T24 cells and allowed to grow for 10 d. Then GW9662 (1 mg/kg) and vehicle were then intraperitoneally injected every other day for additional 14 d. After the mice were killed, tumour tissues were dissected. B, The tumour volume was calculated before each injection, and statistical analysis was carried out with the t test. The days after transplantation of T24 cells and the tumour size are indicated. *P < 0.05, **P < 0.01. C, Representative haematoxylin and eosin staining of tumour tissues dissected from the tumour‐bearing mice. The scale bar is indicated. D, The proliferation of T24 cells treated with GW9662 and vehicle in vivo was measured by immunofluorescence staining of Ki‐67 (green). Nuclei were stained with 4',6‐diamidino‐2‐phenylindole (DAPI; blue), and the scale bar is indicated