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. 2019 Mar 15;38(9):e101140. doi: 10.15252/embj.2018101140

Figure 2. The two‐helix finger switches between two states.

Figure 2

  1. A representative FRET trace (blue line) was obtained as in Fig 1 and fit with a hidden Markov model (black dashed line).
  2. Traces as in (A) obtained in the presence of different nucleotides were used to determine the number of states best fit by the Markov model.
  3. Transition density plot of idealized ATP FRET states obtained in (B).
  4. The distributions of dwell times of the low FRET states observed in ATP were fit with a single exponential (1,500 low FRET states). The inset shows average dwell time and error, defined as the standard error based on the number of traces.
  5. As in (D), but with high FRET (1,656 high FRET states).
  6. Representative traces obtained with ATPγS. The upper FRET trace was calculated from the middle traces obtained by exciting the donor fluorophore and measuring both donor (green) and acceptor (red) fluorescence. The lowest trace was obtained by exciting the acceptor fluorophore directly. The arrow indicates a bleaching event.
  7. Distribution of FRET values determined from 168 traces as in (D) fit with a Gaussian model (black curve).

Source data are available online for this figure.