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. 2019 Apr 1;38(9):e100554. doi: 10.15252/embj.2018100554

Figure EV1. Further characterisation of ATG16L1 lipid binding activity.

Figure EV1

  1. Microscopy‐based protein–liposome binding assay. ATG16L1∆WD40‐GFP or GFP alone was purified from ATG5−/− cells and immobilised on beads followed by incubation in the presence of rhodamine‐labelled liposome preparations containing PI3P. Scale bar: 50 μm.
  2. Quantification of relative liposome binding in (A) along with ATG16L1WT‐GFP from Fig 3E. Quantifications depict means and error bars (SEM) from three independent experiments. ***P ≤ 0.001 (pairwise unpaired Student's t‐test).
  3. Lipid binding experiment using the indicated lipid‐coated beads incubated with recombinant wild‐type ATG16L1 purified from insect cells. Bound proteins were analysed by Western blot using anti‐ATG16L1 antibodies. Right panel shows quantifications depicting means and error bars (SEM) from at least three independent experiments. *P ≤ 0.05 (pairwise unpaired Student's t‐test).