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. 2019 Mar 1;23(5):3495–3504. doi: 10.1111/jcmm.14246

Figure 2.

Figure 2

Effects of glycyrrhizin on apoptosis of ARPE‐19 cells in vitro. A, Hochest 33342 staining of ARPE‐19 cells were treated with sodium iodate at a concentration of 50 μmol. a, sham; b, retinal pigment epithelium (RPE) treated with sodium iodate (SI); c, RPE treated with sodium iodate plus glycyrrhizin (SI + GA). B, Sodium iodate significantly increased apoptosis of ARPE‐19 cells, compared normal control (*P < 0.01), while glycyrrhizin significantly inhibited apoptosis of ARPE‐19 cells treated with sodium iodate, compared with sodium iodate control (# P < 0.01). C, Flow cytometry analysis of apoptosis of ARPE‐19 cells treated with SI at a concentration of 50 μmol. D, Sodium iodate significantly increased apoptosis of ARPE‐19 cells compared normal control (*P < 0.01), while glycyrrhizin treatment significantly decreased the apoptosis of ARPE‐19 cells treated with SI, compared with SI control (# P < 0.01). E, Western blot detection of the effect of glycyrrhizin on cleaved caspase‐3 in RPE treated with sodium iodate. Lane 1, sham; Lane 2, RPE treated with SI; Lane 3, RPE treated with SI + GA. F, Sodium iodate significantly increased expression of cleaved capase‐3 in ARPE‐19 cells compared with normal control (*P < 0.01), while glycyrrhizin significantly decreased the expression of cleaved‐caspase 3 in ARPE‐19 cells treated with sodium iodate, compared with the sodium iodate control (# P < 0.01).