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. 2019 Mar 18;38(9):e100526. doi: 10.15252/embj.2018100526

Figure EV1. Validation of transcriptional changes observed in K14ΔNLef1 keratinocytes.

Figure EV1

  1. Flow‐sorting strategy for basal keratinocytes (Itga6+Cd34−) and bulge stem cells HFSC (Itga6+Cd34+).
  2. Z‐score heat‐map representing DEG between WT‐ or K14ΔNLef1‐sorted all, basal or HFSC keratinocytes. Validation of these results was performed by RT–qPCR. Data are means ± SEM from 3 to 4 mice. (ns) Not significant; *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.00005; ordinary one‐way ANOVA.
  3. Gene Ontology enrichment analysis and network visualization of DEG in HF vs IFE vs. SG (Donati et al, 2017) and WT vs K14ΔNLef1 keratinocyte expression profiles.
  4. Z‐score heat‐map representing the intersection between ΔNLef1 direct target genes (ChIP‐Seq signals ± 3 kb around the peaks centers (C); right panels), DEG in SG vs. IFE and HF (Donati et al, 2017), and WT vs. K14ΔNLef1 keratinocyte expression profiles (obtained in Fig 1I; left panels).
Data information: (B–D) Upregulated genes in K14ΔNLef1 epidermis are shown in red and downregulated genes in blue.