Figure 4.

MiR‐224‐5p/miR‐497‐5p over‐expression increased the expression of ZO‐1, occludin and claudin‐5, and decreased BBB permeability in AD microenvironment. (A, F) Relative mRNA expressions of miR‐224‐5p (A) and miR‐497‐5p (F) in ECs pre‐incubated with Abeta_1‐42, MEM and Abeta_1‐42 + MEM were detected by qRT‐PCR. Data represent mean ± SD (n = 3, each). *P < 0.05, **P < 0.01 vs Control group, ^&& P < 0.01 vs Abeta_1‐42 group, ^# P > 0.05 vs Control group. (B, G) TEER values of Abeta_1‐42‐incubated ECs were detected to confirm BBB integrity. (C, H) HRP flux test was performed to confirm BBB permeability. (D, I) Western blot analysis was used to determine the expression of ZO‐1, occludin and claudin‐5 in Abeta_1‐42‐incubated ECs. Data represent mean ± SD (n = 3, each). **P < 0.01 vs miR‐224‐5p/miR‐497‐5p (+) NC group, ^## P < 0.01 vs miR‐224‐5p/miR‐497‐5p (−) NC group. (E, J) Immunofluorescence assay was used to analyze the expression and distribution of ZO‐1, occludin and claudin‐5. Images were representative of three independent experiments. Scale bar represents 30 μm