Figure EV1. HOIP, HOIL‐1L, and SHARPIN co‐aggregate with Htt‐Q97 (related to Fig 1).

1. HEK293T cells transiently co‐expressing Htt‐Q25 or Htt‐Q97 and either HOIP, HOIL‐1L, SHARPIN, or HHARI were analyzed by the filter retardation assays. Aggregated proteins retained by cellulose acetate membranes were detected by Western blotting.
2. Endogenous HOIP interacts with soluble Htt‐Q97. HEK293T cells were transfected with Htt‐Q97‐HA or Htt‐Q97‐GFP (as a control for specific immunoprecipitation of HA‐tagged Htt‐Q97). Cells were lysed with 1% Triton X‐100, followed by an immunoprecipitation of Htt via the HA tag. Immunoprecipitated proteins were analyzed by Western blotting using an anti‐HOIP antibody.

Source data are available online for this figure.