Figure 2. Linear ubiquitin chains are enriched at Htt‐polyQ aggregates.

• A–C
  M1‐linked ubiquitin co‐localizes with Htt‐polyQ aggregates in cultured SH‐SY5Y cells (A), R6/2 mouse cortex and striatum (B) and human HD frontal cortex (C). Autofluorescent lipofuscin appears in the green channel in human brain. Scale bar, 20 μm (A), 10 μm (B, C).
• D
  Htt‐Q25‐ or Htt‐Q97‐expressing HEK293T were analyzed by filter retardation assays. As indicated, the M1 ubiquitin antibody was preincubated with recombinant tetra‐ubiquitin linked via M1 (4 × M1 ubiquitin) or K63 (4 × K63 ubiquitin).

Data information: See also Fig EV2.Source data are available online for this figure.